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george stark  (Addgene inc)


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    Structured Review

    Addgene inc george stark
    George Stark, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plegfp+wt+stat3/pLEGFP-WT-STAT3+(Plasmid+%2371450)/10__1038_slash_s41598___026___44867___w-58-17-19
    Average 93 stars, based on 21 article reviews
    george stark - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Enriched conditioning expands the regenerative ability of sensory neurons after spinal cord injury via neuronal intrinsic redox signaling
    Article Snippet: .. The SD STAT3 insert was ligated into the expression vector, pEGFP-C1, using T4 DNA Ligase and T4 DNA Ligase Buffer (10×) (New England Biolabs). pLEGFP-WT-STAT3 and pLEGFP-Y705F-STAT3 plasmids were gifts from George Stark (Addgene plasmids #71450 #71445). pLEGFP-WT-STAT3 and pLEGFP-Y705F-STAT3 plasmids were both digested with HindIII to release the 2337 bp mouse STAT3 fragments. ..

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. Glutathione S-transferase (GST) pull-down assays The cDNA fragment encoding full-length human STAT3 was subcloned from pLEGFP-WT-STAT31 into the mammalian expression vector pEBG2 using KpnI and NotI restriction enzyme sites and the following subcloning primer pairs: forward: 5’-GGGGTACCTCGAGCTCAAGCTTCAGGATGG-3’ and reverse: 5’- ATAAGAATGCGGCCGCTCACTTGTAGTCCATGGGGGAGGTA-3’. pLEGFP-WT-STAT3 was a gift from Dr. George Stark (Addgene plasmid # 71450; http://n2t.net/addgene:71450; RRID:Addgene_71450)1. pEBG was generously shared by Dr. David Baltimore (Addgene plasmid # 22227; http://n2t.net/addgene:22227; RRID:Addgene_22227)2. .. Freestyle 293F cells (Thermo Fisher Scientific) grown in FreeStyleTM 293 Expression Medium (Thermo Fisher Scientific; 12338018) in an incubator at 37°C with 8% CO2 were transfected with pEBG-STAT3 plasmid according to the instructions of the Freestyle 293 Expression System (Thermo Fisher Scientific) and then harvested by centrifugation at 1500 x g for 10 minutes.

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. The cDNA fragment encoding full-length human STAT3 was subcloned from pLEGFP-WT-STAT3 ( ) into the mammalian expression vector pEBG ( ) using KpnI and NotI restriction enzyme sites and the following subcloning primer pairs: forward, 5′-GGGGTACCTCGAGCTCAAGCTTCAGGATGG-3′ and reverse, 5′-ATAAGAATGCGGCCGCTCACTTGTAGTCCATGGGGGAGGTA-3′. pLEGFP-WT-STAT3 was Addgene plasmid #71450 ( http://n2t.net/addgene:71450 ; RRID: Addgene_71450; deposited by Dr George Stark) ( ). pEBG was Addgene plasmid #22227 ( http://n2t.net/addgene:22227 ; RRID: Addgene_22227; deposited by Dr David Baltimore) ( ). .. FreeStyleTM 293F cells (Thermo Fisher Scientific) grown in FreeStyleTM 293 Expression Medium (12338018, Thermo Fisher Scientific) in an incubator at 37°C with 8% CO 2 were transfected with pEBG-STAT3 plasmid according to the instructions of the Freestyle 293 Expression System (Thermo Fisher Scientific) and then harvested by centrifugation at 1500 g for 10 min.

    Article Title: Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids.
    Article Snippet: In brief Li et al. demonstrate that FAO mediates chemoresistance in part by acetylating STAT3 through increased acetyl-CoA.. Acetylated STAT3 upregulates long-chain acyl-CoA synthetase 4 (ACSL4).. Upregulated ACSL4 enhances phospholipid synthesis, which is accompanied by elevated mitochondrial membrane phospholipid levels and strengthened mitochondrial membrane potential.

    Plasmid Preparation:

    Article Title: Enriched conditioning expands the regenerative ability of sensory neurons after spinal cord injury via neuronal intrinsic redox signaling
    Article Snippet: .. The SD STAT3 insert was ligated into the expression vector, pEGFP-C1, using T4 DNA Ligase and T4 DNA Ligase Buffer (10×) (New England Biolabs). pLEGFP-WT-STAT3 and pLEGFP-Y705F-STAT3 plasmids were gifts from George Stark (Addgene plasmids #71450 #71445). pLEGFP-WT-STAT3 and pLEGFP-Y705F-STAT3 plasmids were both digested with HindIII to release the 2337 bp mouse STAT3 fragments. ..

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. STAT3 cDNA (human α isoform, residues 1-770) was subcloned from pLEGFP-WT-STAT3 ( ) into the RGS-6xHis-pcDNA3.1 plasmid , which was Addgene plasmid #52534 ( http://n2t.net/addgene:52534 ; RRID: Addgene_52534; deposited by Dr Adam Antebi) ( ). .. The 6xHis-STAT3α fragment was cloned into the pFastBacTM Dial vector, generously provided by Dr George Aslanidi at The Hormel Institute, University of Minnesota.

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. Glutathione S-transferase (GST) pull-down assays The cDNA fragment encoding full-length human STAT3 was subcloned from pLEGFP-WT-STAT31 into the mammalian expression vector pEBG2 using KpnI and NotI restriction enzyme sites and the following subcloning primer pairs: forward: 5’-GGGGTACCTCGAGCTCAAGCTTCAGGATGG-3’ and reverse: 5’- ATAAGAATGCGGCCGCTCACTTGTAGTCCATGGGGGAGGTA-3’. pLEGFP-WT-STAT3 was a gift from Dr. George Stark (Addgene plasmid # 71450; http://n2t.net/addgene:71450; RRID:Addgene_71450)1. pEBG was generously shared by Dr. David Baltimore (Addgene plasmid # 22227; http://n2t.net/addgene:22227; RRID:Addgene_22227)2. .. Freestyle 293F cells (Thermo Fisher Scientific) grown in FreeStyleTM 293 Expression Medium (Thermo Fisher Scientific; 12338018) in an incubator at 37°C with 8% CO2 were transfected with pEBG-STAT3 plasmid according to the instructions of the Freestyle 293 Expression System (Thermo Fisher Scientific) and then harvested by centrifugation at 1500 x g for 10 minutes.

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. The cDNA fragment encoding full-length human STAT3 was subcloned from pLEGFP-WT-STAT3 ( ) into the mammalian expression vector pEBG ( ) using KpnI and NotI restriction enzyme sites and the following subcloning primer pairs: forward, 5′-GGGGTACCTCGAGCTCAAGCTTCAGGATGG-3′ and reverse, 5′-ATAAGAATGCGGCCGCTCACTTGTAGTCCATGGGGGAGGTA-3′. pLEGFP-WT-STAT3 was Addgene plasmid #71450 ( http://n2t.net/addgene:71450 ; RRID: Addgene_71450; deposited by Dr George Stark) ( ). pEBG was Addgene plasmid #22227 ( http://n2t.net/addgene:22227 ; RRID: Addgene_22227; deposited by Dr David Baltimore) ( ). .. FreeStyleTM 293F cells (Thermo Fisher Scientific) grown in FreeStyleTM 293 Expression Medium (12338018, Thermo Fisher Scientific) in an incubator at 37°C with 8% CO 2 were transfected with pEBG-STAT3 plasmid according to the instructions of the Freestyle 293 Expression System (Thermo Fisher Scientific) and then harvested by centrifugation at 1500 g for 10 min.

    Article Title: A critical role for STAT3 Thr714 phosphorylation in NPM-ALK-driven tumorigenesis
    Article Snippet: Antibodies against β-actin and Lamin B were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). .. The cDNA encoding NPM-ALK was inserted into the MSCV-IRESGFP retroviral vector [8]. pLEGFP-WT-STAT3 was a gift from George Stark (Addgene plasmid # 71450 ; http://n2t.net/addgene:71450 ; RRID:Addgene_71450). .. The threonine-to-alanine substitution at residue 714 of STAT3 (T714A) was generated using a site-directed mutagenesis kit (Stratagene, CA).

    Article Title: Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids.
    Article Snippet: In brief Li et al. demonstrate that FAO mediates chemoresistance in part by acetylating STAT3 through increased acetyl-CoA.. Acetylated STAT3 upregulates long-chain acyl-CoA synthetase 4 (ACSL4).. Upregulated ACSL4 enhances phospholipid synthesis, which is accompanied by elevated mitochondrial membrane phospholipid levels and strengthened mitochondrial membrane potential.

    Subcloning:

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. Glutathione S-transferase (GST) pull-down assays The cDNA fragment encoding full-length human STAT3 was subcloned from pLEGFP-WT-STAT31 into the mammalian expression vector pEBG2 using KpnI and NotI restriction enzyme sites and the following subcloning primer pairs: forward: 5’-GGGGTACCTCGAGCTCAAGCTTCAGGATGG-3’ and reverse: 5’- ATAAGAATGCGGCCGCTCACTTGTAGTCCATGGGGGAGGTA-3’. pLEGFP-WT-STAT3 was a gift from Dr. George Stark (Addgene plasmid # 71450; http://n2t.net/addgene:71450; RRID:Addgene_71450)1. pEBG was generously shared by Dr. David Baltimore (Addgene plasmid # 22227; http://n2t.net/addgene:22227; RRID:Addgene_22227)2. .. Freestyle 293F cells (Thermo Fisher Scientific) grown in FreeStyleTM 293 Expression Medium (Thermo Fisher Scientific; 12338018) in an incubator at 37°C with 8% CO2 were transfected with pEBG-STAT3 plasmid according to the instructions of the Freestyle 293 Expression System (Thermo Fisher Scientific) and then harvested by centrifugation at 1500 x g for 10 minutes.

    Article Title: Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeability
    Article Snippet: .. The cDNA fragment encoding full-length human STAT3 was subcloned from pLEGFP-WT-STAT3 ( ) into the mammalian expression vector pEBG ( ) using KpnI and NotI restriction enzyme sites and the following subcloning primer pairs: forward, 5′-GGGGTACCTCGAGCTCAAGCTTCAGGATGG-3′ and reverse, 5′-ATAAGAATGCGGCCGCTCACTTGTAGTCCATGGGGGAGGTA-3′. pLEGFP-WT-STAT3 was Addgene plasmid #71450 ( http://n2t.net/addgene:71450 ; RRID: Addgene_71450; deposited by Dr George Stark) ( ). pEBG was Addgene plasmid #22227 ( http://n2t.net/addgene:22227 ; RRID: Addgene_22227; deposited by Dr David Baltimore) ( ). .. FreeStyleTM 293F cells (Thermo Fisher Scientific) grown in FreeStyleTM 293 Expression Medium (12338018, Thermo Fisher Scientific) in an incubator at 37°C with 8% CO 2 were transfected with pEBG-STAT3 plasmid according to the instructions of the Freestyle 293 Expression System (Thermo Fisher Scientific) and then harvested by centrifugation at 1500 g for 10 min.

    Retroviral:

    Article Title: A critical role for STAT3 Thr714 phosphorylation in NPM-ALK-driven tumorigenesis
    Article Snippet: Antibodies against β-actin and Lamin B were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). .. The cDNA encoding NPM-ALK was inserted into the MSCV-IRESGFP retroviral vector [8]. pLEGFP-WT-STAT3 was a gift from George Stark (Addgene plasmid # 71450 ; http://n2t.net/addgene:71450 ; RRID:Addgene_71450). .. The threonine-to-alanine substitution at residue 714 of STAT3 (T714A) was generated using a site-directed mutagenesis kit (Stratagene, CA).

    Transfection:

    Article Title: Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids.
    Article Snippet: In brief Li et al. demonstrate that FAO mediates chemoresistance in part by acetylating STAT3 through increased acetyl-CoA.. Acetylated STAT3 upregulates long-chain acyl-CoA synthetase 4 (ACSL4).. Upregulated ACSL4 enhances phospholipid synthesis, which is accompanied by elevated mitochondrial membrane phospholipid levels and strengthened mitochondrial membrane potential.

    FLAG-tag:

    Article Title: Fatty acid oxidation protects cancer cells from apoptosis by increasing mitochondrial membrane lipids.
    Article Snippet: In brief Li et al. demonstrate that FAO mediates chemoresistance in part by acetylating STAT3 through increased acetyl-CoA.. Acetylated STAT3 upregulates long-chain acyl-CoA synthetase 4 (ACSL4).. Upregulated ACSL4 enhances phospholipid synthesis, which is accompanied by elevated mitochondrial membrane phospholipid levels and strengthened mitochondrial membrane potential.



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